Remember me on this computer. Enter the email address you signed up with and we'll email you a reset link. Need an account? Click here to sign up. Download Free PDF. A short summary of this paper. None of the 42 patients with IgG2 deficiency whom we studied has a history of vasculitis. We have also studied 56 patients with vasculitis, and found four of them with IgG2 levels below the normal range for their age. As a group, the vasculitis patients did not differ in the incidence of low IgG2 levels from a normal population.
Three of the four vasculitis patients with low IgG2 levels had a Henoch-Sch6nlein syndrome, and the association between both features was statistically significant. In most cases and whenever IgG1 and IgG3 can mediate a carbohydrate-specific antibody the clinical features were not determinant of a particular type of response in young children Shackleford et al.
Immunological determina- IgG subclass deficiencies particularly IgG2, and to a lesser tions were made at the time the vasculitis was diagnosed. These include chronic respiratory vasculitis, when the patient was not undertaking therapy, infections Heiner, , severe diarrhoea Oexelius, and obtaining similar results.
Forty-two sera of healthy blood intractable epilepsy of childhood Duse et al. More donors were measured as controls. After an for this study. A serum pool calibrated against WHO stan- Font-n, The Netherlands was added in the appropriate dilution.
Jimenez, M. Fontan Table 1. Clinical features of patients with IgG2 Table 2. There are no statistically significant the following sources: clone HP was a kind gift of Dr 0. Jefferies University of presented any of the autoantibodies studied.
Birmingham, UK. None of the immunodeficient patients affected by IgG2 defi- ciency had a history of vasculitis. Thus, the DNA, Crithidia luciliae.
Rheumatoid factor was determined by incidence of IgG2 deficiency in the vasculitis population could laser nephelometry Beckman. Anti-cardiolipin antibodies be explained, at least partially, by the 'natural' incidence of that were measured by a modification of a previously described deficiency in normal populations; we also found that IgG2 ELISA technique Loizou et al.
The x2 test was used to Peachey, IgG2 deficiency in our patients seems to be compare the data between different groups. Our adult patients were diagnosed not only on clinical grounds, but also by means of skin biopsy, showing the None of the 40 patients with confirmed IgG2 deficiencies had typical leukocytoclastic vasculitis characteristic of this entity.
In vasculitis. A short summary of this paper. The method was based upon competitive tem with antibodies coupled to a solid phase. Ferritin was purified from human method suitable for the assay of ferritin in human serum in spleen by a modification of the method described by Drys- clinical studies. The preparation was kindly For personal use only. This preparation was used for immunization, serum mean Patients for labelling with lZ5Iand as a provisional reference stand- with iron overload or hepatic damage had high values, as ard preparation.
Three different antisera were com- in iron deposits during phlebotomy and iron treatment in pared. Two antisera were raised in two rabbits by weekly 3 healthy men. The animals were bled 3 weeks after the last injection.
Labelling of ferritin. To mg of p-hydroxyphenyl- In an immunoradiometric assay for ferritin in propionic acid N-hydroxysuccinimide ester was added serum was described by Addison et al. The labelled product was puri- sorbent for ferritin antibody, labelling of the anti- fied by chromatography on a Sephadex G column.
Even if not all workers find the iodine were obtained. Radioimmunoassays ferritin antibodies. Solidphase coupling of anti-ferrifin. For direct coupling for the assay of different antigens. Ferritin has been of antibodies 5 to 10 p1 of the antisera were coupled to labelled with lZ5Iby the chloramine-T technique but mg of CNBr-activated ultrafine Sephadex or micro- only to a very low specific activity Attempts crystalline cellulose as previously described Anti- to label ferritin by that method to high specific ac- bodies were also indirectly coupled 19 , i.
The mg and antifemtin-antibodies were then bound to present work presents a method for measuring fer- the coupled ferritin. The particles were suspended in ritin in serum by using ferntin labelled with a p - assay buffer solution. Wide and G. Serum samples were obtained from the fol- lowing subjects: 25 male and 33 female medical students 90 years old. Blood donors were excluded as well as pregnant women.
Thirty-seven male blood donors, ran- 80 domly selected, were investigated. Three healthy young males were 2 c' 50 phlebotomized once weekly until they were anaemic and - iron depleted, after which parenteral iron treatment was P LO given in a dose not quite sufficient to restore the haemo- Z 30 globin level to the initial values.
From this experiment samples were selected before phlebotomy, at the time of lowest haemoglobin level and after iron treatment. Logit representation of the dilution curve for the used in the assays for at least 4 months.
The anti- For personal use only. The three anti- sera could be used for assays per ml Assay buffer solution. The esti- 0. The pH mated ferritin concentration in serum was not sig- of the final solution was adjusted to 7.
The assays were technically per- the three antisera were compared, testing serum formed as previously described for the assay of gonado- tropins All samples were run in duplicate.
The Sepha- test serum was incubated with 1 ml of the solid phase dex particles gave a slightly better precision than antibody suspension. The amount of polymer-coupled microcrystalline cellulose. After 3 4 h of incubation at room temperature the labelled ferritin 40 cpm was added in 0.
I ml of assay versus 0. After washing three times with saline with O. Quality control data were obtained from nine as- The values given are expressed in arbitrary units per litre, says over a period of 4 months which included obtained by giving the geometric mean for a population duplicate tests on a total of serum samples. The of 25 normal young men no blood donors , age years, the value of The results of the assay and dif- average percentage of labelled ferritin bound to B" ferent quality control parameters were calculated using a was The intra-assay variation calcu- Gel chromatography.
C Fig. The highest value iation from nine repeated assays of control samples recorded, arb. Her bone arb. One patient with pure The dose-response curve for serial dilutions of red cell aplasia, who had had several blood trans- the reference standard, diluted in 2.
A fresh serum sample from a The ferritin levels in serum during induction of healthy young male 80 arb. U of ferritin per litre anaemia and after iron treatment of 3 healthy young was chromatographed on a Sephadex G column men is shown in Fig. Ferritin fell to a subnormal and all fractions were assayed for ferritin content.
Two immunoreac- marrow hemosiderin could be detected. When iron tive peaks eluted soon after the void volume were detected Fig. This elution pattern for immuno- Ferritin I reactive ferritin was almost identical with that ob- tained for the radioactivity when 'labelledferri- tin added to normal serum was gel chromato- graphed on the same Sephadex G column.
The levels of ferritin in serum in normal males and females and male blood donors is shown in Fig. The geometric mean for males was given the value of arb. Serum ferritin levels in normal males, females The 4 patients with liver cirrhosis had values of and male blood donors.
On gel chromatography of serum, the im- There was no difference in relative The relation 90i between mean ferritin concentration in serum from Fedin healthy men and women was similar to that re- ported by other investigators 1 , 4 , 8, 9, Male blood donors had significantly lower mean ferritin concentrations than other healthy males of the same age. The results of the clinical material and so the variation in immunoreactive ferritin concentra- Ups J Med Sci Downloaded from informahealthcare.
Mean Hb concentrations and serum ferritin levels assay. Several types of ferritin have been isolated in 3 healthy young men during phlebotomy and iron treat- from various tissues in man 6, 10 and some of ment.
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